br To see how the quantity of osteosarcoma in

To see how the quantity of osteosarcoma in vitro studies developed overtime, we set out to systematically identify all the drugs that have been tested on osteosarcoma CHC in vitro in the past two decades. A PubMed search strategy was compiled which can be found in Supplementary data 1. To our surprise, we got a total of 5282 hits in PubMed. It struck our attention that the number of publications on treatment of osteosarcoma cell lines with various compounds has increased almost exponentially (Fig. 1A). After importing these PubMed hits in EndNote X7, we obtained an indication of how the amount of publications developed in China, the USA, India, Australia, Canada and Europe (defined as countries that are in the Schengen Area and/or the European Union) in the past few years by searching for these countries in the authors address box. The majority of the publications came from China, Europe or the USA. The amount of publications from the USA showed a minor increase, from 45 in 1996 to 73 in 2015, whereas the amount of publication from Europe increased substantially from 35 in 1996 to 176 in 2015. Strikingly, the amount of publications from China rose from 1 to 359, thereby mainly being responsible for the huge increase in publications observed in the last few years. China has been the leading country since 2012, and 53% of the publications from 2015 involved Chinese institutes; this is a much higher proportion than reported for other research areas such as haematology research [4]. We read all the abstracts of the hits of the past three years (n=1755), and categorized them based on non-osteosarcoma (n=297, many studies on other cancers metastasizing to the bone), no in vitro study (n=179), no drugs study (n=596, many studies performed siRNA knockdown only) and no inhibitory effect (n=126), leaving us with 560 studies from the last three years, a relatively large number when considering the rareness of this disease. Interestingly, approximately 1/3 of the compounds could be classified as traditional medicine, of which over 80% was published by Chinese authors. For example, we encountered several compounds such as Evodiamine, a chemical extracted from the Tetradium genus of plants [5,6] and crude extracts of Rheum palmatum L., the root of Chinese rhubarb [7].
The use of a single cell line Remarkably, many studies were performed with only one cell line, either U-2 OS or MG-63. Due to the high heterogeneity of osteosarcoma, studying a panel of cell lines instead of a single cell line is essential for eventual clinical applicability. U-2 OS and MG-63 are both ATCC cell lines established in 1964 and 1977 respectively. Searching PubMed for U-2 OS (and U2OS/U2-OS/U-2-OS) and MG-63 (and MG63) identified respectively 1449 and 2564 publications from 1996 to 2015, while the combination only resulted in 163 PubMed hits. Determining the amount of publications per year and per country for these cell lines as described above further demonstrated the almost exponential increase in publications with osteosarcoma cell lines attributable to studies from Chinese institutes (Fig. 1B). To compare these results with another ATCC sarcoma cell line with a comparable amount of total publications, we performed an identical search for HT-1080 (fibrosarcoma), in which we did not see this trend (Fig. 1B), demonstrating that this is not a trend observed in all ATCC sarcoma cell lines. U-2 OS and MG-63 are ubiquitously applied also outside the osteosarcoma field for general cell biology studies because they are among the few human cell lines that are relatively susceptible to transfection. In addition, these cells grow rapidly facilitating experimental application but also boosting drug responsiveness [8]. This probably explains why so many in vitro studies have been published for this relatively rare disease.
A researcher\'s perspective There may be a jewel hidden in this avalanche of studies, but it is difficult to discern due to the huge quantity of papers using questionable designs. If in vitro studies are performed, researchers should always include a panel of cell lines to represent the tumour heterogeneity in osteosarcoma patients. As a researcher, it is impossible to keep track of all the research that is being published, which may lead to missing promising therapeutic targets. The publication of these relatively easy executable studies is obviously fed by the incentive of “publish or perish”, but it keeps scientists occupied with often irrelevant work, it usurps financial budgets and obscures relevant investigations. Other major drivers of the enormous increase in research papers is the substantial increase in the number of academic journals [9]. Aggressive editor\'s requests to submit manuscripts to new journals keep filling a scientist\'s mailbox, which can be quite disturbing. This trend to prevail quantity over quality is occupying precious time from editors and reviewers. Currently, the Science Citation Index is used for medical career evaluation in the majority of large Chinese hospitals, resulting in a huge pressure of Chinese medical doctors to publish articles and an increase in number of publishing scientists [10,11]. Therefore, it is essential that the evaluation system on research performance will be changed with the focus shifted from quantity to quality as was recently again advocated that bibliometrics are warping science [12]. Our analysis of in vitro osteosarcoma studies illustrates the effect of the increased publication pressure since the convenience of osteosarcoma cell lines renders them into low hanging fruit but results in studies with limited scientific value, which constrains solutions for this deadly disease that affects young patients.