In the current study we compared

2018-10-20

In the current study, we compared two different genetic backgrounds on the induction and maintenance of multipotency by generating clonal iNSC lines from two distinct mouse strains (C57BL/6 vs. C3H). Clonal iNSC lines from distinct genetic backgrounds exhibited the distinct levels of conversion effi

Induction of EndMT results from integration of

2018-10-20

Induction of EndMT results from integration of multiple extrinsic inputs within the dynamically shifting cellular environment of cardiogenesis. Owing to the membrane bound presentation of ligands, Notch signaling status is dependent on the organization and distribution of signal-presenting and signa

The karyotype of the BIOT EHMT iPSC line

2018-10-20

The karyotype of the BIOT-0708-EHMT1 iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). In parallel, the pathogenic NM_024757.4(EHMT1):c.3413G>A mutation was confirmed by Sanger sequencing in the newly established iP

Also critical to the success of hard tissue engineering stra

2018-10-20

Also critical to the success of hard tissue engineering strategies is the selection and use of an appropriate growth factor regimen. Although several growth factors are reported to play an important role in the process of matrix mineralisation, IGF-1 is the most abundant growth factor in bone matrix

Previously we showed that the

2018-10-20

Previously, we showed that the transcription factor SLUG is an important regulator of mammary epithelial lineage commitment and differentiation (Proia et al., 2011). Recent studies have also shown that SLUG is necessary for the mammary stem cell state (Guo et al., 2012). However, SLUG-deficient mice

Another remarkable observation of this study is that

2018-10-20

Another remarkable observation of this study is that ASCL1-iN calcifediol are exclusively excitatory. This is surprising, because ASCL1 is not typically associated with the excitatory neuronal lineage during neural development (Bertrand et al., 2002; Fode et al., 2000; Guillemot et al., 1993; Johns

Traditionally the effects of radiation on

2018-10-20

Traditionally, the effects of radiation on spermatogonia have been evaluated by morphological analyses. It is considered that the percentage of seminiferous tubule cross-sections that show type A spermatogonia 10 days after irradiation is representative of SSC survival (van der Meer et al., 1992). A

To test whether transgenic Cre mouse lines are

2018-10-20

To test whether transgenic Cre mouse lines are capable of specific targeting APCs in vivo, mice carrying the Cre recombinase-responsive Enhanced Yellow Fluorescent Protein (EYFP) allele in the Rosa locus (Rosa-EYFP) (Srinivas et al., 2001) were crossed to one of three Cre lines. These lines were cho

Direct cell reprogramming technology is

2018-10-20

Direct cell-reprogramming technology is based on the dominant action of cell-lineage transcription factors (TFs) in converting adult somatic cells into different cell types (Graf and Enver, 2009). This technique represents a promising avenue in the field of regenerative medicine, with the potential

Existing treatments for myelomeningocele repair consist

2018-10-20

Existing treatments for myelomeningocele repair consist of pre- or post-natal surgical closure of the defect. Prenatal intervention has been shown to be more effective than postnatal surgery because it can prevent or ameliorate sequels (Adzick et al., 2011; Adzick et al., 1998; Meuli et al., 1997; M

To reprogram patient cells we adopted the most feasible and

2018-10-20

To reprogram patient cells, we adopted the most feasible and safest protocol using a patient cell source that is easy to obtain, PB-MNCs, and an integration-free reprogramming strategy based on SeV vectors. PB-MNCs were chosen, as blood collection is common in patient follow-up and is minimally inva

Recently we found that vesicular monoamine transporter VMAT

2018-10-20

Recently, we found that vesicular monoamine transporter 2 (VMAT2) regulates monoamine (including dopamine) storage by acting as a negative regulator, such that high monoamine levels inhibit the progression from Pdx1+ pancreatic progenitor Nanaomycin A to Neurog3+ endocrine progenitor cells of ESC-de

order GDC-0879 Our study made use of single cell transcripti

2018-10-20

Our study made use of single-cell transcriptional profiling to define the heterogeneity of hormone-producing order GDC-0879 derived from hPSCs. The technology is a powerful tool that allowed us to determine the percentage of various hormone-producing cells and shifts in this distribution upon treatm

FOXP labels striatal precursors and differentiated MSNs Dell

2018-10-20

FOXP1 labels striatal precursors and differentiated MSNs (Delli et al., 2013). Diverse studies thus far have attempted to pre-differentiate stem cycloheximide solubility into transplantable striatal progenitors or MSNs by using various kinds of differentiation protocols. Human ESCs were demonstrate

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2018-07-29