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On arrival her vital signs were stable Laboratory
2018-11-06

On arrival, her vital signs were stable. Laboratory data showed normal hemoglobin levels, low platelet counts (66,000/μL), elevated international normalized ratio (1.29), and elevated total bilirubin (1.9 mg/dL). A triphase abdominal CT was applied. In the arterial phase (Figure 3A), a single 16-cm
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To determine the time required for accurate viability assess
2018-11-06

To determine the time required for accurate viability assessment, approximately 1000 A. caninum eggs were transferred into 1.5mL centrifuge tubes, suspended in 1mL Phosphate Buffer Saline (PBS), and heat-inactivated on a heating block for 30min at 80°C. Tubes were centrifuged at 800× for 3min, and t
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Time lapse images were taken on
2018-11-06

Time-lapse images were taken on an inverted Zeiss LSM 510 microscope using Plan-Neofluar 20× (0.8 N.A.) objective. For Ca analysis, images were taken every 1.5s for 3min using 512×512 pixel resolution and cells were excited using the 488nm laser line. On each cell soma a 5μM×5μM ROI was drawn and ch
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In this study besides the high hits for the evolutionarily
2018-11-06

In this study, besides the high hits for the evolutionarily hypervariable sites, we also noticed that certain mtDNA variants were shared by family members, pedigree-specific occurrence of mtDNA variants. This observation was unexpected but confirmed our previous speculation for a family-specific occ
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Although CVB are a frequent
2018-11-06

Although CVB are a frequent cause of subclinical infection, they may also cause other diseases such as viral myocarditis (Pallansch and Roos, 2001), symptoms of which may be seen either shortly after acute infection potentially triggering severe myocardial dysfunction, or during the chronic phase of
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TGF type I receptor inhibitors were also found to induce
2018-11-06

TGF-β type I receptor inhibitors were also found to induce differentiation of insulin-producing cells. Among the TGF-β type I receptor inhibitors that we tested, Alk5 inhibitor II showed unique effects on the differentiation of pancreatic endocrine cells. Only Alk5 inhibitor II significantly increas
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br Results br Discussion Here we describe
2018-11-06

Results Discussion Here, we describe a multistage process for highly efficient nonintegrative cellular reprogramming coupled to a method of scalable hiPSC culture that utilizes stage-specific media formulations: FRM for enhanced reprogramming, and FMM for long-term maintenance. Previous studie
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br Results br Discussion The goal of this study was
2018-11-06

Results Discussion The goal of this study was to define a quality-control standard rubric for assessing stem cell-derived cardiac myocytes. We chose a set of experimental measurements that provide insight into not only the expression profile of the bupropion hydrochloride but also morphologic
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In Huber et al reported the
2018-11-06

In 2007, Huber et al. reported the generation of stable transgenic human embryonic stem cell lines for identification and selection of differentiating CMs. Although the authors used the human MLC-2v promoter reporter construct, they did not characterize the atrial- or ventricular-like features of th
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Introduction The experimental induction of teratoma for
2018-11-06

Introduction The experimental induction of teratoma (for definition of terms see Box 1) in mammals, mostly mice, has been carried out for decades (Evans and Kaufman, 1981; Skreb et al., 1971; Solter et al., 1970; Stevens, 1958, 1970; Stevens & Little, 1954). In stem cell research and banking (ISCBI
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br Materials and methods br Results br Discussion Following
2018-11-06

Materials and methods Results Discussion Following the demonstration that a ROCK inhibitor such as Y27632 permits the survival of single-cell hESCs (Watanabe et al., 2007), several groups utilized it to establish suspension culture systems for the expansion of hPSCs based on single-cell ino
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Although Sca is another conserved marker present
2018-11-06

Although Sca-1 is another conserved marker present in both stromal and epithelial SCs of many tissues, including hematopoietic AP1903 manufacturer (Spangrude et al., 1988), mouse incisor pulp progenitor cells (Balic et al., 2010), lung (Kim et al., 2005), prostate (Lawson et al., 2007), and pancrea
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It has been reported that uremic conditions cause
2018-11-06

It has been reported that uremic conditions cause defects in BMSC self-renewal and differentiation (He et al., 2009; Noh et al., 2012). Our previous studies showed that chronic kidney disease induced by partial nephrectomy (PNx) increases the circulating level of Na/K-ATPase ligands in rat and mouse
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Following from our prior research to
2018-11-06

Following from our prior research to derive new hESC lines under increasingly defined culture conditions (Fletcher et al., 2006) we sought to establish a facility within which our experience and evolving practice and reagents could be implemented to a GMP and professionally accredited standard. Here
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CSCs are identified either by surface markers and up regulat
2018-11-06

CSCs are identified either by surface markers and up-regulation of transcription factors/other genes involved in stem cell pathways or by functional assays to characterize stem like properties like drug efflux, quiescence and self-renewal (Moserle et al., 2010). Studies have reported enrichment of C
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